ATCC® Number:  CCL-243™        Designations:  K-562  Depositors:   HT Holden  Biosafety Level: 1  Shipped:  frozen  Medium & Serum:  See Propagation  Growth Properties: suspension Organism: Homo sapiens ��human��  Morphology: lymphoblast

 Source: Organ: bone marrow Disease: chronic myelogenous leukemia ��CML�� Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.   Applications: transfection host ��Nucleofection technology from Lonza�� Tumorigenic: Yes  Reverse Transcript: negative  Antigen Expression: CD7 ��25%�� DNA Profile ��STR��: Amelogenin: X CSF1PO: 9,10 D13S317: 8 D16S539: 11,12 D5S818: 11,12 D7S820: 9,11 THO1: 9.3 TPOX: 8,9 vWA: 16 Cytogenetic Analysis: The stemline chromosome number is triploid with the 2S component occurring at 4.2%. Fifteen markers ��M1 ��nd M��15���� occurred in nearly all S metaphases. Spontaneous non-specific dicentrics occurred, but rarely. Unstable markers were also rarely seen. The X was disomic, ��nd N9 was nullisomic. Isoenzymes:  AK-1, 1 ES-D, 1 G6PD, B GLO-I, 2 Me-2, 0 PGM1, 0 PGM3, 1 Age:  53 years  Gender:  female  Comments: The continuous cell line K-562 was established by Lozzio ��nd Lozzio from the pleural effusion of a 53-year-old female with chronic myelogenous leukemia in terminal blast crises. [22609] The cell population has been characterized as highly undifferentiated ��nd of the granulocytic series. [26059] Studies conducted by Anderson, et al., on the surface membrane properties led to the conclusion that the K-562 was a human erythroleukemia line. [26060] The K-562 cell line has attained widespread use as a highly sensitive in vitro target for the natural killer assay. [48829] [1101] [48830] See Pross, et al. for a detailed analysis of the in vitro assay of NK cells including the mathematics of quantitation of NK cell activity. [48833] K-562 blasts are multipotential, hematopoietic malignant cells that spontaneously differentiate into recognizable progenitors of the erythrocytic, granulocytic ��nd monocytic series. [26061] The effect of inducers on sublines derived from the original K-562 cell line have been reviewed by Koeffler ��nd Golde. [867] Cultures from the ATCC stock have been shown to exhibit this sensitivity for assessing human natural killer activity. Karyological studies on various K-562 sublines have been classified into three groups ��A,B,C�� by Dimery, et al. [26063] The strain obtained by the ATCC most closely resembles the B population. Occurrence of the Philadelphia chromosome, however, was of much lower frequency; none detected in 15 metaphases examined. The line is EBNA negative. Propagation:  ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Iscove��s Modified Dulbecco��s Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.Atmosphere: air, 95%; carbon dioxide ��CO2��, 5% Temperature: 37.0°C Subculturing:  Protocol: Cultures can be maintained by the addition or replacement of fresh medium. Start new cultures at 1 X 10 exp5 viable cells/ml. Subculture at 1 X 10 exp6 cells/ml. Medium Renewal: Every 2 to 3 days Preservation:  Freeze medium: Complete growth medium 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor temperature Related Products: Recommended medium ��without the additional supplements or serum described under ATCC Medium��:ATCC 30-2005recommended serum:ATCC 30-2020purified DNA:ATCC CCL-243Dpurified RNA:ATCC CCL-243R References: 867: Koeffler HP, Golde DW. Human myeloid leukemia cell lines: a review. Blood 56: 344-350, 1980. PubMed: 69967651101: Ortaldo JR, et al. Specificity of natural cytotoxic reactivity of normal human lymphocytes against a myeloid leukemia cell line. J. Natl. Cancer Inst. 59: 77-82, 1977. PubMed: 6903622609: Lozzio CB, Lozzio BB. Human chronic myelogenous leukemia cell-line with positive Philadelphia chromosome. Blood 45: 321-334, 1975. PubMed: 16365826059: Lozzio BB, Lozzio CB. Properties ��nd usefulness of the original K-562 human myelogenous leukemia cell line. Leuk. Res. 3: 363-370, 1979. PubMed: 9502626060: Andersson LC, et al. K562����a human erythroleukemic cell line. Int. J. Cancer 23: 143-147, 1979. PubMed: 36797326061: Lozzio BB, et al. A multipotential leukemia cell line ��K-562�� of human origin. Proc. Soc. Exp. Biol. Med. 166: 546-550, 1981. PubMed: 719448026063: Dimery IW, et al. Variation amongst K562 cell cultures. Exp. Hematol. 11: 601-610, 1983. PubMed: 657690932357: Chan YJ, et al. Two distinct upstream regulatory domains containing multicopy cellular transcription factor binding sites provide basal repression ��nd inducible enhancer characteristics to the immediate-